![]() Every time that I call them, it takes 3-5 days to get my account manger or someone to call me back to tell me someone else will call me back in 3-5 business days. When we ask for a refund, they tell us that it needs an active account to set-up the POS system. NOTHING IS WORKING YET AFTER 3 MONTHS! We have been paying 3 monthly payment already without anything working. We signed up on Jand promised that the set-up will take about 1-2 weeks. ![]() Their Support Team takes time to resolve problems, rather than rushing us offline to keep up with some productivity metric.(Did I say I LOVE Lightspeed?!!)ĪVOID THIS COMPANY IF POSSIBLE! Horrible customer services! Our sales rep was extremely fast and responsive by calling us every day to follow up until the day we made the first payment. She sent me the steps to fix the problem, stayed with me while the changes were made, and then followed up later to ensure everything was working properly. we had made a late night keystroke mistake entering the updated prices!What caught our eye with Lightspeed was the powerful Back-of-House infrastructure that allows us to make REMOTE CHANGES to our menus, our controls etc IN AN INSTANT on our phones, tablets or computers: last year on a trip to China, we got a text from one of our managers requesting a menu addition - somewhere over the Pacific at 40,000 feet, my wife added the item and went back to sleep!!!! The level of detail that supports our business goes far beyond what we could imagine - while a lot of detail & thought has to go into setting up Lightspeed initially, that effort pays dividends when using the system.Finally, the support we get from the Technical Support team is amazing - the Techs are all very well trained, are extremely knowledgeable and can resolve problems that are outside their Lightspeed purview: in September, a store was having a weird connectivity problem that was traced by Catherine in Tech Support back to a DHCP problem with our Wi-Fi (nothing to do with Lightspeed). philosophy until a sharp eyed customer got very upset because she noticed the price of one of our specialty coffees at one location was vastly different from another location. ![]() When we bought the business in 2016, we installed another companys system which, after a few months use, revealed its weakness for use in a multi-unit business.We plodded along for awhile under the Better to stay with the Devil you know. This is our second year of using Lightspeed. We operate 4 Cannonball Cafés in Jamaica. would be better if it had a are you sure and a red warning.All of this aside, the inventory, labor management, reporting, POS, Work Order, and PO to SO tracking, makes this so much easier.Chris WhiteStore ManagerWheel Sport Bikes Spokane, WA Lastly, if you sell an item, not in inventory, there is only a yellow warning, at the top of the screen. So if you are trying to search for a just a labor type, you have to select labor, every line. It uses a shared search, for all of the different tabs. It works, but could use some streamlining.2. Building a Special order, and transferring it, is clunky, and hard to train staff on. Lots of small customizable menus gives a range of flexibility.Some cons, to make this a honest review.1. Super stable, in 2 years there have only been 2 times the server went down, and both were fixed in under 10 minutes.We have 4 stores, and with a shared inventory, it has made things simpler. Support is also a key, and they have been great. The ability to use a POS from anywhere, on most platforms is amazing. I have used Lightspeed in a bike shop and it has been absolutely key to success.
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Wait for the font generator to provide you with different styles. 104k Members 133 Online Created Join Powerups Powerup to unlock perks for r/identifythisfont Become a Hero Community Heroes Powerups 6/25 nihilist_hippie 1 theantivirus 1 Alt4Norm 1 Mattyboy808 1 Anonymous Heroes 2Type your text into the font generator. ![]() You're all set! A Subreddit for Identifying Fonts: show us a sample and we'll try to find the font. Type the name of the font, click it so a checkmark is next to the name, then click OK at the bottom of the menu. First, click the dropdown arrow next to the font menu and tap more fonts at the top. You'll have to add most of these fonts to Google Docs, but this doesn't take much time or effort to do. You can then save the image, or use the EMBED button to get image links. You can use the following tool to generate text graphics based on your selection of colors, text effects and sizes using futuristic fonts in seconds. Identified font: Neuropol.If you just want to create text graphics and logos online using futuristic fonts, you can check out our text generator below. Será que teria como vocês encontrarem essa fonte,é para um trabalho de design, por favor. 364,878 downloads (6 yesterday) 2 comments Free for personal use - 2 font files.Fonts 1 - 10 of s 1990s futuristic techno headline science fiction square display sans serif decorative computer medium wide bold science heavy 1960s modern space technology canadian 1970s retro electronic scientific high tech narrow 2020s text 2030s sport military industrial 1980s metaverse angular funny rounded italic ultramodern fatY2K Aesthetic font #1. Hope you enjoy and learn something from t.Y2K Neophyte. Dragon Ball, the greatest shonen fight manga's logo in Y2K style.This logo/artwork was made inspired by the series. Press question mark to learn the rest of the keyboard shortcuts View on Libreddit, an alternative private front-end to Reddit. help identify a variety of y2k fonts hello, hope you can help me with this one in the black and white picture i think the bottom left one is Bjork but i could be wrong, didnt have a.809 members in the Y2K community. 2) Copy and paste the result from the below result. 1500+ unique aesthetic text appears below the input box and you can simply copy and paste with a one-click on your favourite style. Use code "KYBER20” to get 20% off 5 or more fonts! This tool is fully user friendly and simple, anyone can use this web application just enter the text in the input box. SmoothSwing fonts designed for CFX, Proffie, and Verso. ![]() Press question mark to learn the rest of the keyboard shortcutsFonts 1 - 10 of s 1990s futuristic techno headline science fiction square display sans serif decorative computer medium wide bold science heavy 1960s modern space technology canadian 1970s retro electronic scientific high tech narrow 2020s text 2030s sport military industrial 1980s metaverse angular funny rounded italic ultramodern fatSmoothSwing fonts designed for CFX, Proffie, and Verso. It’ll look good advertising rollerblades and skateboards or as the logo for a tech website destroyed by the dot-com bubble. Browse by alphabetical listing, by style, by author or by popularity. Press question mark to learn the rest of the keyboard shortcuts Each time you take the feat, it applies to a different spell. Special: You can gain this feat multiple times. This increases the minimum level of the prepared spell or spell slot you must sacrifice in order to cast it but does not affect the casting time. You can apply any metamagic feats you possess to this spell when you cast it. You can cast that spell spontaneously by sacrificing a prepared spell or spell slot of equal or higher level. Prerequisites: Spellcraft 5 ranks, Heighten Spell.īenefit: Choose one spell which you have the ability to cast. You find it very easy to cast one particular spell. Preferred Spell Source Advanced Player's Guide pg. Metamagic Master) to allow the creature to prepare in (or cast using) a 2nd-level slot an intensified empowered shocking grasp spell.Achievement | Alignment | Armor Mastery | Betrayal | Blood Hex | Called Shot | Combat | Combination | Conduit | Coven | Critical | Damnation | Esoteric | Faction | Familiar | Gathlain Court Title | Grit | Hero Point | Item Creation | Item Mastery | Meditation | Metamagic | Monster | Origin | Panache | Performance | Shield Mastery | Stare | Story | Style | Targeting | Teamwork | Trick | Weapon Mastery | Words of Power This means that the benefit of the trait Magical Lineage can be combined with the benefit of the trait Wayang Spellhunter (i.e. However, the benefits of the traits Magical Lineage and Waywang Spellhunter are not bonuses-and even different benefits-, so the two traits can work in conjunction. For example, the trait Elven Reflexes and the trait Reactionary each grant a +2 trait bonus (the bonus's type) on initiative checks so a creature with both traits gains only a +2 trait bonus on initiative checks. …But the benefits of Magical Lineage and Waywang Spellhunter can be combinedĮxcept in rare case, bonuses of the same type or from the same source don't stack, and, instead, only the highest bonus of the same type is applied. There's no Metamagic Master trait in the Dragon Empires Primer as per its provenance on the d20PFSRD, only Wayang Spellhunter. Although both are regional traits and many GMs do ignore the rule that says that a creature can't take more than one trait from each trait category (see here on Restrictions on Trait Selection), the traits are also the same trait: The d20PFSRD renamed and changed the description of the trait Metamagic Master to remove product identity from the trait Wayang Spellhunter. It's unlikely that a GM will allow one creature to take both the Metamagic Master trait and the Wayang Spellhunter trait. Metamagic Master and Wayang Spellhunter are the same trait… Arma incantata Magus con archetipo Myrmidarch, VMC paladin, advanced weapon training Warrior Spirit, Gloves of dueling +2. By the way, this spell should be recallable with a Pearl of Power (1st).ĭo Magical Lineage, Metamagic Master and Wayang Spellhunter stack together? Metamagic master/Wayang spellhunter -1, tratto. ![]() If you’re looking for the most optimized wizard possible, you’ll probably want to do one of three builds, the conjurer, the evoker, or the diviner. So a 1st level Intensified Empowered Shocking Grasp. Answer (1 of 3): How you want to design your wizard depends a lot on what you want to do. Deeply spiritual, they follow a philosophy known as The Dissolution, which teaches that in passing they may again merge into the shadow. ![]() They are extremely gaunt, with pixielike stature and skin the color of deep shadow. If they stack, I could cast a Shocking Grasp with Intensified Spell (+1) Empower Spell (+2) with Magical Lineage (-1), Metamagic Master (-1) and Wayang Spellhunter (-1). The wayangs are a race of small supernatural humanoids who trace their ancestry to the Plane of Shadows. When you use this spell with a metamagic feat, it uses up a spell slot one level lower than it normally would. You grew up on one of the wayang-populated islands of Minata, and your use of magic while hunting has been a boon to you. Most clutches stick to one of these models to determine their leader when members of one clutch come into conflict over which exact one, the victor is allowed to determine the clutch's new definition of strength. Strength can be interpreted as anything from physical might to magical talent and shrewd scheming. When you use the chosen spell with a metamagic feat, it uses up a spell slot one level lower than it normally would. Strength and dominance are the two core values of xulgath society. When you apply metamagic feats to this spell that add at least 1 level to the spell, treat its actual level as 1 lower for determining the spell’s final adjusted level. Pick one spell when you choose this trait. ![]() Good processing speed in large projects.A spread of features needed for editing animations.Possibility of output in various formats like SWF film or slide.Compatible with different formats in entries.Range of colors for designing and applying custom colors.Quite 60 fancy and proprietary font models.Aurora 3D Animation Maker creates limitless special animation. Using just a single timeline, you will be able to control all aspects of your animation, including the length, playback speed, and more. It lets you create awesome 3D animations with your text or logos. There are over 60 professionally prepared designs within the software. Aurora 3D Animation Maker is easiest 3D title and banner creation software.There are 3000 models of Ready Object types.Or apply motion effects like Linear, Quad, Cubic, and Quit to add choreography like bounce and twists. Imagine applying particle effects to simulate fireworks, smoke, snow, flame, or the emptiness of space. Attractive and user-friendly interface. Aurora 3D Animation Maker creates limitless special animation effects, as far as your creativity can go, to further liven up your creations.Animation design to be used in banners. ![]()
The safest solution is to see your dermatologist where they can be destroyed via laser treatment or electrodesiccation, which is an in-office preventative skin cancer treatment where a professional uses tools to scrape the affected skin's surface.Īs many as 40 percent of adults are affected by keratosis pilaris (opens in new tab)-hard, little red bumps that usually appear on the backs of your upper arms and thighs-which is caused by a buildup of excess keratin in the skin follicle, blocking the pore. Even though the world of skincare tutorials on YouTube is amazing doesn't mean we should try everything at home. ![]() When it comes to dealing with these small spots, don't take matters into your own hands. There's nothing itchy about these guys, but that doesn't mean you can go ahead and mess with them! Bleeding can occur if the angioma is scratched, rubbed, or cut open which is never ideal. They are usually the size of any freckle you might have already on your body, but they can be bigger. Also, wear long-sleeved shirts and pants, and make sure to check your body for ticks afterward.Cherry angiomas, basically a fancy name for red moles, are a collection of small blood vessels that are usually found on your torso, arms, legs, and shoulders if you're 30 or older. Use insect repellant in grassy areas and the woods to prevent tick bites.Protect your skin from sun damage with clothing, sunscreen and shade.Don’t share items that may have touched someone else’s mouth or nose (like a cup or toothbrush).Clean countertops, door handles and other high-touch surfaces frequently.But you can help prevent infections that lead to petechiae with some simple strategies: It’s not possible to prevent all causes of petechiae. Nonsteroidal anti-inflammatory drugs (NSAIDs).Some home remedies that may help include: If you have petechiae, you should call your doctor. What can I do at home to treat petechiae? Corticosteroids to reduce swelling in the blood vessels.Chemotherapy, radiation, immunotherapy or bone marrow transplant to treat leukemia.Antibiotics to treat a bacterial infection.If there is a more serious cause, you may need: For straining or a skin injury, you may not need any treatment. Treatment for petechiae varies depending on the cause. Other signs include swollen gums, achy joints, easy bruising and shortness of breath. Vitamin C deficiency: When your body doesn’t get enough vitamin C, you can develop scurvy.Other symptoms may include high fever, easy bruising or bleeding, body aches and weakness. Viral hemorrhagic fevers: Viral hemorrhagic fevers, such as Ebola and dengue fever, make it hard for the blood to clot.It also causes fever, headache, weight loss and nerve problems (pain, weakness or numbness). Vasculitis: Vasculitis is inflammation (swelling) in the blood vessels.It may also cause easy bruising, bloody noses or gums, blood in pee or poop, and yellowish skin and eyes. Thrombocytopenia: With thrombocytopenia, you have low levels of platelets, which help your blood clot.Examples include when you’re throwing up, lifting something very heavy or giving birth. Straining: When you strain, you can break blood vessels under the skin.It often causes fatigue, headache, sore throat, swollen glands and tonsils, and fever. Mononucleosis: Also called mono, this viral infection is common among young people.Medications: Some medications may cause petechiae, including certain antibiotics, antidepressants and medications that thin the blood.Other signs of this disease may include weight loss, swollen glands, easy bleeding or bruising, nosebleeds and night sweats. Leukemia: Leukemia is cancer in the blood and bone marrow.Examples include a car accident, bite, friction on the skin or even sunburn. Injury: Damage to the skin can cause petechiae.Other signs of infection may include fatigue, fever, sore throat, swollen glands and tonsils, body aches, nausea and vomiting. So can viral infections, such as cytomegalovirus or hantavirus. Infection: Illnesses from bacteria, such as strep throat with scarlet fever, or Rocky Mountain spotted fever (spread by ticks) can cause petechiae.Other signs include fever, chills, fatigue, body aches and shortness of breath. Endocarditis: Endocarditis is an infection in the lining of the heart.Several things can lead to petechiae, ranging from simple and reversible causes to serious illnesses: What are the possible causes of petechiae? ![]() ![]() (Yeah, it really looks like the screenshots.)Feedback**********************************Thanks for your awesome response and feedback. Metal support and better Battery consumptionWatch the preview video now to see real gameplay of 5-vs-5 multiplayer battles. ” –PocketGamer PodCastCustom Game Engine**********************************Game now plays well with all Devices including devices with smaller app size and memory footprint. MaskGun is a casual real-time Multiplayer First Person Shooter built for Touch.Cross-platform 3D Multiplayer FPS game for IOS and Android with a new realtime presence system allows you play with your friends by joining them in a game.Three Game modes - DeathMatch, Team DeathMatch and Bomb Defusal Mode.Three Arena Maps – Yard, GhostTown and Factory.Mask - Express yourself using MasksGun - Choose between 16 Primary Weapons or Three Secondary Weapons Armor - Custom Armor choicesPatterns and Decals – Customize Guns and ArmorFeatures**********************************- Free to Play as much as you want, no energy system - Realtime Friends Presence system - New Loadout to customize Gun, Armors and Masks with Patterns and Decals - Three new Modes including DeathMatch, Team Death Match and Bomb Defusal - Three new Maps including Yard, Ghosttown and Factory - Complete Missions to Level Up - Party Mode Up with friends.- New Game Modes and Maps coming soonAwards for MaskGun**********************************NGDC Studio Game of the Year 2016 Praise for MaskGun**********************************“One game that I saw that is going to do exceptionally well in our community and in the App Store, is MaskGun” – Eli Hodapp, Editor - TouchArcade, Podcast“I quite like the first person shooter with the Masks. Using a script or add-on that scans GameFAQs for box and screen images (such as an emulator front-end), while overloading our search engine. ![]() There is no official GameFAQs app, and we do not support nor have any contact with the makers of these unofficial apps. Continued use of these apps may cause your IP to be blocked indefinitely. ![]() This triggers our anti-spambot measures, which are designed to stop automated systems from flooding the site with traffic.
![]() I'll go retail if I can't get it online (Kid Icarus or Street Fighter 4), I think I'll enjoy it but eventually sell/trade it when I'm done (Super Mario Land 3D), it's cheaper in the store (used or otherwise, preferably by a margin of more than just a few bucks coughGameStopcough), or it has a cool collector's edition. I'll download if I think I'm going to play it for the life of the machine (Mario Kart 7 or Brain Age, something with staying power, you know?) or the price is compelling enough (Tetris Axis: $10 online vs $15-20 retail). It all depends on the game itself and the price. On topic, I'm open to either download or retail for full-priced games. Swapnote actually told me to delete notes today because I'm out of room and couldn't download anymore. Right now, music is taking up half of it (StreetPass and the pedometer work when you're listening to music with the system closed and headphones in). I'm using the same 2gb card that came with the system. Well, I've been thinking about upgrading my card anyway. £4! Nintendo want almost as much for a VC Gameboy game. They're Windows only and I have no Windows PC, so I can't even play them, but they cost my a combined total of £4, and now they'll be waiting there ready for the day I do end up with a Windows PC. Heck, I bought Max Payne, Max Payne 2 and Fable: The Lost Chapters last week. It's so convenient to have these games in your library, and because they're so cheap. Almost everyone I know buys far more games on Steam than they will ever be able to play, for the above two reasons. They're making a huge effort to push eShop downloads, but instead of a big marketing campaign they just need to get the basics right. They could probably drop it to £20 and STILL be making more profit than physical copies. The dumbest part is if they charged £25 for a download, they'd still make a lot more profit on that, than what they are for the physical copies. Animal Crossing pre-order £25 at Tesco, Donkey Kong £27 at ShopTo, Fire Emblem was £27 at ShopTo etc, but Nintendo want £39.99 for all these on the eShop. It's easy enough to use a price checker ad change your price to match. If they took the step of making sure every big retail game was no more expensive than the physical version, then it would be a no brainer and I'd download everything. If my digital purchases were backed up and could be re-downloaded to any machine I was logged into it, then I'd pay a lot more digital stuff from Nintendo. It's almost like they don't know how to do it, but that sounds ridiculous. I don't understand why Nintendo are being so stubborn over the unified account thing. Not having all those boxes cluttering up your home is wonderful, but you also don't have to go hunting for discs and books, and they're going to last forever unlike physical media which can be lost, damaged, or even just degrade (there's plenty of evidence for CDs kept in cases for 10 years and then no longer working when they're taking out due to chemical degradation). I'd love to go digital only, just like I have for music, books, movies and PC games. Nintendo are shooting themselves in the foot. ![]() His store has been neglected… by neglected, I mean that his manager is still on payroll as she has been on six month sick leave in Hawaii. MMC is the eldest pickle bro and in a competition with his other bro’s to take the family pickle franchise over. The first quarter of this book could’ve and should’ve been condensed to ONE chapter at most. It often takes an entire page to convey one thought of little substance. His store has been neglected… by neglected, I mean that his manager is still on Ehhh, by 25%, I haven’t laughed once, which isn’t a good indicator for a ROM-COM. (Dang it, I did it there.) Please take a look at her book, because it's as funny and sweet and hysterical as she is.moreĮhhh, by 25%, I haven’t laughed once, which isn’t a good indicator for a ROM-COM. She's one of those people who, if you're in pickle, you call her. (She also writes under the pen name Deanna Roy.) She's one of the nicest people I know. I could make all kinds of puns in here about how Jace is one spicy pickle, or this book is a big dill! but the book is just funnier than anything I'm going to come up with. The problem? Smack-talking Nova Strong, who is either everything wrong with his deli, or everything right for him.īig Pickle is one of the cutest, spiciest books that I've read in ages. I totally needed a funny, spicy book like Big Pickle. Big Pickle is one of the cutest, spiciest books that I've read in ages. The problem? Smack-talking Nova Strong, who is either everything wrong with his deli, or everything right for him. Is Nova Strong everything wrong with my deli, or everything right for me?īig Pickle is a full-length, 300-page standalone romantic comedy full of pickle jokes, giggle-worthy references to male anatomy, and more salty language than a jar of baby dills.more She has no idea I’m only there to spy.Īs I get to the bottom of why my franchise is failing, I desperately need to know one thing: She’s perfectly happy to call bullsh*t when she sees it.īut despite the way we nearly melt the permafrost in the industrial freezer, I have to control myself. I hightail it to Texas to see what’s happened to my restaurant, only to find a sassy little spitfire named Nova Strong in charge. And we have the best salami.īut when my father announces the franchise will go to the most profitable of the three brothers, I learn that even though I’m the biggest Pickle, I’m dead last. I happen to be the oldest Pickle in the family deli business. But when my father 7 hours and 34 minutesĪn enemies-to-lovers, secret boss, gut-splittingly hilarious rom com from USA Today bestselling author JJ Knight (Although that’s true too.) I happen to be the oldest Pickle in the family deli business. An enemies-to-lovers, secret boss, gut-splittingly hilarious rom com from USA Today bestselling author JJ Knight They call me Big Pickle. He has done many advertising campaigns for many varied brands such as Coca-Cola, Dish Network, Ford, hhgreg, Lowe's and others.7 hours and 34 minutes He’s kind of a big dill. In 2014 he won the recognition of Miami Mayor Tomas Pedro Regalado for his contribution to the art featured in the work "Mesalina" in the leading role as John. He has appeared in many TV soap operas, for example Al ritmo de tu corazón in which he played Santiago Duque (a leading role) Milagros de Amor, Corazón valiente and others. ![]() He began his acting career in a reality show called Protagonistas de Novela in 2002, in which he emerged as the winner. Jaider Villa Giraldo (born 30 January 1977), better known as Jaider Villa, is a Colombian actor, host and model. įANCJ has also been reported to have FA pathway-independent functions and has in particular been implicated in the resolution of secondary structures, most notably G4 structures. FANCJ was shown to be part of the downstream factors, but–somewhat surprisingly–its function in the FA pathway seems to be independent of the interaction with the HR factor BRCA1, whereas it depends on the interaction with the mismatch repair protein MLH1 and a functional helicase domain. FA proteins work together in the so-called FA pathway that promotes ICL repair by the interplay of lesion excision, translesion synthesis and homologous recombination (HR). Fanconi anemia is a chromosome instability and cancer-prone disorder that has so far been linked to mutations in 21 genes. Later on, biallelic mutations in FANCJ were found to be cause of disease in a subset of Fanconi anemia (FA) patients. The latter finding is important since a number of cancer-associated mutations are located within the iron-sulfur domain of FANCJ.įanconi anemia group J protein (FANCJ) was initially identified as an interaction partner of breast cancer type 1 susceptibility protein (BRCA1) and therefore termed BRCA1-interacting protein 1 (BRIP1) or BRCA1-associated C-terminal helicase 1 (BACH1). Mechanistically, FANCJ’s ability to resolve G4 structures largely depends on an intact helicase domain and partially on the iron-sulfur cluster-binding domain. Combined, our findings support a model whereby FANCJ resolves G4 structures in the context of the replisome to allow replication through guanine-rich regions of the genome. Our data further suggest that single amino acid changes in FANCJ that abolish its helicase activity or its ability to bind an iron-sulfur co-factor are sufficient to render cells more sensitive to CX-5461 treatment. In this study we show that FANCJ-deficient cells have an increased sensitivity towards CX-5461. It was originally identified as an rDNA transcription inhibitor, but–more recently–also found to bind and stabilise G4 DNA secondary structures. The small molecule CX-5461 is currently in phase I/II clinical trials for patients with BRCA-deficient tumours. Mutations in FANCJ that abolish FeS cluster binding may hence be predictive of an increased cellular sensitivity towards G4-stabilising agents.īreast and ovarian cancers are often linked to a genetic predisposition, most commonly through mutations in the breast cancer susceptibility genes BRCA1 and BRCA2, but also other genes, such as FANCJ/BRIP1, are associated with an increased disease risk. On the other hand, FANCJ knock-out cells expressing FeS cluster-deficient variants display a similar–enhanced–sensitivity towards pyridostatin (PDS) and CX-5461, two agents that stabilise G4 structures, as FANCJ knock-out cells. ![]() This may suggest a partially retained cellular activity of FANCJ variants with alterations in the FeS domain. Surprisingly, however, FANCJ variants that are unable to bind an FeS cluster and to unwind DNA in vitro can partially suppress the formation of replisome-associated G4 structures that we observe in a FANCJ knock-out cell line. Moreover, we find that FANCJ requires an intact FeS cluster for its ability to unfold G4 structures on the DNA template in a primer extension assay with the lagging-strand DNA polymerase delta. Here we show that the FeS cluster is indispensable for FANCJ’s ability to unwind DNA substrates in vitro and to provide cellular resistance to agents that induce ICLs. ![]() Several cancer-associated mutations are located in the FeS domain of FANCJ, but how they affect FeS cluster binding and/or FANCJ activity has remained mostly unclear. Mutations in FANCJ are associated with Fanconi anemia and an increased risk for developing breast and ovarian cancer. ![]() FANCJ/BRIP1 is an iron-sulfur (FeS) cluster-binding DNA helicase involved in DNA inter-strand cross-link (ICL) repair and G-quadruplex (G4) metabolism. |
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